Abstract
The growth of plantlets in Temporary Immersion Bioreactor system (TIBs) relies on initial successful liquid phase transition process. The response of N. diderrichii explants was assessed in liquid-M Smedia with a view to mass produce its seedlings using TIBs. Seven treatments consisting (A) 0.0/0.0, (B) 0.0/0.1, (C) 0.1/0.0, (D) 0.2/0.1, (E) 0.3/0.2, (F) 0.4/0.3 and (G) 0.5/0.4mg/lBAP/NAA combinations were studied. Each group consist of seven replicates and group A without Growth Regulators (GR) serves as control. The results at 4 Weeks after Inoculation (WAI) showed that effects of the growth regulators were significant on shoot length and number of adventitious shoots while number of roots and leaves were closely related. Treatment E produced highest number of adventitious shoots (3.6) which was higher than 0.9 shoots from treatment G and closely related to others. Maximum number of leaves (16.6) was produced by treatment F followed by E (15.7) while the least (12) was obtained in treatment A. The highest number of roots (4.9) was obtained from treatments B, followed by E (4.3) with the lowest being recorded in C (2.43). Liquid MS medium supplemented with 0.3/0.2mg/lBAP/NAA shows some promise for plantlets generation for the purpose of multiple shoot production of N. diderrichii in TIBs.
Highlights
The growth of plantlets in Temporary Immersion Bioreactor system (TIBs) relies on initial successful liquid phase transition process
The results of the analysis of variance of the data collected showed that treatment effects was significant (p ≤ 0.05) for shoot length and number of adventitious shoots while that of number of leaves and roots were not significant (p 0.05) at 2 and 4 weeks after inoculation (WAI).The medium without Growth Regulators (GR) gave the highest shoot length of 2.86 cm and was significantly higher than other treatments at 2 Weeks after Inoculation (WAI) (Table 1)
By 4 WAI, the same medium devoid of GR produced 4.0 cm shoot length which was comparable to 3.14 cm from medium added 0.4/0.3 mg/l Benzyl Amino Purine (BAP)/Naphtalene Acetic Acid (NAA) while higher than others (Table 1 and Plate 1).In addition, media with 0.0/0.1 and 0.3/0.2 mg/l BAP/NAA produced comparable average number of adventitious shoots of 2.1 each at 2WAI (Table 1)
Summary
The growth of plantlets in Temporary Immersion Bioreactor system (TIBs) relies on initial successful liquid phase transition process. Highest average number of roots of 3.7 and 4.9 were obtained from medium added 0.0/0.1 mg/l BAP/NAA at 2 and 4 WAI respectively (Figure 2).
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