In Vitro Development of Meristematic Centers in Cyclea peltata—A Pharmaceutically Important Plant

Abstract

The callus derived from nodal explant of Cyclea peltata on Murashige Skoog medium supplemented with α-naphthalene acetic acid + benzyl amino purine (5.4 μM + 0.44 μM) was used for indirect shoot proliferation. The stem derived calli of 50–60 d old were inoculated to half strength and full strength Murashige Skoog media with different growth regulators at varying concentrations. The callus, upon subculture to the medium supplemented with either naphthalene acetic acid or indole acetic acid alone or in combination with cytokinins, led to the development of shiny, small, globular cells. Histological studies revealed the induction of callus from the cambial cells of the explant and the presence of numerous, actively dividing, darkly stained meristematic centers in the organogenic callus.