Abstract
The objective was to assess the ability of testicular spermatozoa to fertilize in vitro matured domestic cat oocytes and support blastocyst formation in vitro following intra-cytoplasmic sperm injection (ICSI). After IVM, oocytes were randomly and equally allocated among treatment groups (ICSI with testicular spermatozoa, ICSI with ejaculated spermatozoa, sham ICSI, and control IVF). At 18 h after either injection or insemination, the percentage of fertilized oocytes (per total metaphase II oocytes) was ∼65% after ICSI with testicular or ejaculated spermatozoa ( P > 0.05), which was less ( P < 0.05) than control IVF (∼90%). On Day 7, the percentage of cleaved embryos (per total metaphase II oocytes) was ∼60% after ICSI with testicular or ejaculated spermatozoa ( P > 0.05), which also was less ( P < 0.05) than control IVF (∼85%). After ICSI with testicular spermatozoa, the percentage of blastocysts (per total cleaved embryos) was ∼11.0%, which was less ( P < 0.05) than ICSI with ejaculated spermatozoa (∼21.0%); the latter was less ( P < 0.05) than control IVF (∼43.0%). No blastocyst formation was observed after sham ICSI. For the first time in the domestic cat, this study demonstrated the fertilizing ability and developmental potential of intra-testicular spermatozoa delivered directly into intra-ovarian oocytes matured in vitro.
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