In Vitro Detoxification of Aflatoxin B1, Deoxynivalenol, Fumonisins, T-2 Toxin and Zearalenone by Probiotic Bacteria from Genus Lactobacillus and Saccharomyces cerevisiae Yeast

Katarzyna Śliżewska,Agnieszka Chlebicz

doi:10.1007/s12602-018-9512-x

Katarzyna Śliżewska, Agnieszka Chlebicz

Open Access

https://doi.org/10.1007/s12602-018-9512-x

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Abstract

The aim of the following research was to determine the detoxification properties of probiotic Lactobacillus sp. bacteria (12 strains) and S. cerevisiae yeast (6 strains) towards mycotoxins, such as aflatoxin B1, deoxynivalenol, fumonisins, T-2 toxin and zearalenone, which pose as frequent feed contamination. The experiment involved analysing changes in concentration of mycotoxins in PBS solutions, after 6, 12 and 24 h of incubation with monocultures of tested microorganisms, measured by high-performance liquid chromatography (HPLC). We found that all strains detoxified the mycotoxins, with the highest reduction in concentration observed for the fumonisin B1 and B2 mixture, ranging between 62 and 77% for bacterial strains and 67–74% for yeast. By contrast, deoxynivalenol was the most resistant mycotoxin: its concentration was reduced by 19–39% by Lactobacillus sp. strains and 22–43% by yeast after 24 h of incubation. High detoxification rates for aflatoxin B1, T-2 toxin and zearalenone were also observed, with concentration reduced on average by 60%, 61% and 57% by Lactobacillus, respectively, and 65%, 69% and 52% by yeast, respectively. The greatest extent of reduction in the concentration for all mycotoxins was observed after 6 h of incubation; however, a decrease in concentration was noted even after 24 h of incubation. Thus, the tested microorganisms can potentially be used as additives to decrease the concentrations of toxins in animal feed.

Highlights

Mycotoxins are secondary metabolites with low molecular mass (~ 700 Da), which are synthesised by filamentous fungi, belonging mostly to the Ascomycota phylum
The biological material included potential probiotic bacteria of Lactobacillus genus and strains of the yeast S. cerevisiae, which are deposited in the Łódź Collection of Pure Cultures 105 of Institute of Fermentation Technology and Microbiology at Technical University of Łódź (Table 2)
Bacteria belonging to the Lactobacillus genus were characterised by their diverse ability to detoxify aflatoxin B1

Summary

Introduction

Mycotoxins are secondary metabolites with low molecular mass (~ 700 Da), which are synthesised by filamentous fungi, belonging mostly to the Ascomycota phylum. Moisture content within 16–30%, high temperature reaching 25–30 °C, and high relative air humidity (80–100%) are conditions that stimulate growth of filamentous fungi and mycotoxin production [10]. The concentration of toxins in input materials (e.g. corn, grass, clover) is not reduced to a sufficient degree while they are being processed into feed, as these metabolites are resistant to high and low temperatures, even after long storage period [9, 11]. These toxins constitute a threat, as Probiotics & Antimicro. European Union legislation specifies tolerable daily intake (TDI) for a variety of mycotoxins, in addition to providing guidance values for their concentrations in animal feedstuffs (Table 1) [18]

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