In Vitro Detection of Some Active Compounds in Stressed Callus of Mungbean (Vigna Radiata L.)

Abstract

An experiment was conducted at the Ministry of Sciences and Technology/Directorate of Agricultural Research, Genetic Engineering Department. In Vitro callus cultured on (MS) medium supplemented with different levels of NaCl, brassinolides and vitamin C. H.P.L.C technique was used to detect the phenolic compounds in different treatments of mungbean stressed callus. The results showed various responses for accumulation of phenolic compounds in different treatments of mungbean stressed callus for example, 15dS.m−1NaCl+40mg.L−1vitamin C+0.04mg.L−1brassinolide) accumulated highest concentration of Hydroxybenzonic acid, Tannic acid, Gallic acid, Quercetin and Syrinigic acid (280.44, 135.16, 286.49, 778.93 and 778.93 μg.ml−1) respectively, while highest accumulation of Chlorogenic acid, Gentisic acid, Sikimick acid and Coumaric acid were found in (6dS.m−1NaCl+40mg.L−1vitamin C+0.04mg.L−1brassinolide) treatment reached (230.27, 208.21, 846.52 and 522.62 μ.mg−1)respectively. Furthermore, 6dS.m−1NaCl+0.04mg.L−1 brassinolide and 15dS.m−1 NaCl+0.04mg.L−1 brassinolide achieved highest accumulation (933.83 and 407.48 μg.ml−1) for Ferulic acid and Protocatechuric acid respectively.