In vitro demonstration of intestinal absorption mechanisms of different sugars using 3D organotypic tissues in a fluidic device_suppl

Alessandra Marrella

doi:10.14573/altex.1908311s

Abstract

In vitro demonstration of intestinal absorption mechanisms of different sugars using 3D organotypic tissues in a fluidic device_suppl

Highlights

The intestinal epithelium is the main access point of nutrients and orally administered drugs to the systemic circulation (Ganapathy, 2012)
Animal-based tests reproduce the dynamic stimuli of the intestinal environment mediated by the systemic blood circulation, it is impossible to selectively distinguish and decouple all the variables involved in the passage of molecules through the intestinal barrier in human intestinal tissues (Cao et al, 2006)
Cells were obtained from commercial sources or isolated from tissues obtained from the International Institute for the Advancement of Medicine (IIAM; Scranton, PA) through the Organ Procurement Organization (OPO)

Summary

Introduction

The intestinal epithelium is the main access point of nutrients and orally administered drugs to the systemic circulation (Ganapathy, 2012). Animal-based tests reproduce the dynamic stimuli of the intestinal environment mediated by the systemic blood circulation, it is impossible to selectively distinguish and decouple all the variables involved in the passage of molecules through the intestinal barrier in human intestinal tissues (Cao et al, 2006). For these reasons, there is a growing interest to develop advanced, human-relevant in vitro models, where key parameters such as intestinal permeability and tissue barrier properties can be controlled and monitored at low cost and reduced time

Methods

Results

Conclusion