In-vitro degradation behavior and biocompatibility of superhydrophilic hydroxyapatite coating on Mg–2Zn–Mn–Ca–Ce alloy

Abstract

Membranes of guided bone regeneration (GBR) are widely used in oral implant surgeries. This study successfully developed a new GBR membrane, hydroxyapatite (HA) coating, on Mg–2Zn–Mn–Ca–Ce alloy substrates via a hydrothermal technique. The features of the HA coatings were investigated by complementary characterization methods (SEM, XRD, EDS, and static contact angle). The in-vitro degradation behavior and biocompatibility of HA-coated and bare Mg–2Zn–Mn–Ca–Ce alloys were analyzed in detail. The HA coatings exhibited porous outer surface and compact inner layer, outstanding hydrophilicity, and thus significantly improved the anticorrosion/biodegradability of the Mg alloy substrate in Hank's solution. Moreover, the results of CCK-8 assays and cell adhesion tests suggested that HA-coated samples could further promote cell adhesion and proliferation. HA-coated Mg–2Zn–Mn–Ca–Ce alloys have good biodegradation behavior and biocompatibility, indicating great potential as a GBR membrane material.