In vitro cytotoxicity of Artocarpus lakoocha aqueous extract and oxyresveratrol in SH-SY5Y cells

Abstract

Artocarpus lakoocha is a traditional plant found in South/Southeast Asia, which has been used for centuries as antelmenthic in form of an aqueous extract. This extract, also called puag haad in Thailand, contains oxyresveratrol, a stilbene, as a major active compound of puag haad. To evaluate biological activity of these tested compounds, cytotoxicity study was conducted to determine proper doses of the tested compounds in SH-SY5Y neuroblastoma cells. SH-SY5Y cells are a neuroblastoma cell line which can be used to study several neurodegenerative diseases in cell cultural models. This study aimed to examine cytotoxicity of A. lakoocha aqueous extract and oxyresveratrol in SH-SY5Y neuroblastoma cells by XTT and LDH assays. The result of this study demonstrated that ≤25µg/ml of puag haad and ≤200 µM of oxyresveratrol did not show toxicity to SH-SY5Y neuroblastoma cells. The concentrations at ≤25µg/ml of puag haad and ≤200 µM of oxyresveratrol could be used to investigate neuroprotective effect using SH-SY5Y neuroblastoma cells. Further studies are required to investigate the effect of A. lakoocha aqueous extract and oxyresveratrol related to neurodegenerative disease in cell culture system