In vitro Cytotoxicity examination of the seashell and fish scale substances embedded with high degree of compactness in polyethylene composite

Abstract

The organic response of polymer composites is facilitated by adding natural filler materials. Several new composite formulations claim to reduce the environmental impact. The present study assessed the cytotoxic responses of seashell and fish scale filled composite materials. The Polyethylene high-density (PEHD), 30 wt. % of the seashell and 30 wt. % of a seashell and fish scale combinations blended with the polymer matrix composites with 10:3 proportion (PEHD 100 g:Reinforcements 30 g), and are then fabricated by the plastic injection molding machine. To better imitator the microstructure and the mineral component of natural bone, novel hydroxyapatite / polymer composite scaffolds are allowed to interact 10, 20, 30, 40 and 50 μl of standard fresh cell culture medium for 24 hours. Morphology of cell, cell viability, and the effect of Cytotoxicity on polyethylene based composite samples were examined through ISO 10993:5, and 10993:12 test standards. The percentage of cell viability and the level of toxicity were compared.