In Vitro Cytotoxicity and Risk Assessments of Environmental Pollutants Using Fibroblasts of a Stranded Finless Porpoise (Neophocaena asiaeorientalis).

Abstract

Cetaceans accumulate high levels of environmental pollutants, yet their toxicological studies have been difficult due to technical and ethical issues. It is essential to identify and fill the current knowledge gaps in the in vitro assays available for cetaceans. The present study establishes a novel in vitro assay that uses the fibroblasts of a finless porpoise (Neophocaena asiaeorientalis) (FF) stranded in the Seto Inland Sea (SIS) to answer questions about the cytotoxicity and risks of environmental pollutants. FF were treated with 17 compounds including polychlorinated biphenyls (PCBs) and dichlorodiphenyltrichloroethane and their metabolites (DDTs) and evaluated for cytotoxicity, viability, and apoptosis. The results of FF were compared with those of human fibroblasts (HF). The relative potencies of the test compounds were comparable between the two species, as EC50 of these compounds significantly correlated for FF and HF. Exposure-activity ratios (EARs) revealed that accumulation of PCBs and DDTs are likely to pose adverse effects at the cellular level in the SIS finless porpoises, as their tissue concentrations exceeded EC50 values obtained in this study. This study successfully evaluated the risks of environmental pollutants using cetacean fibroblasts isolated by a non-invasive method that may be applied to various cetacean species and compounds.