In vitro cytotoxic effect of PARP inhibitor alone and in combination with nab‑paclitaxel on triple‑negative and luminalA breast cancer cells.

Abstract

In the present study, the invitro cytotoxic effect of poly(ADP‑ribose) polymerase(PARP) inhibitor alone and in combination with nab‑paclitaxel was evaluated on human triple‑negative breast cancer(TNBC) cell line MDA‑MB‑231 and human luminalA breast cancer cell line MCF‑7. For this purpose, cell index(CI) values obtained from xCELLigence Real‑Time Cell Analysis(RTCA) DP instrument, mitotic index(MI), labelling index(LI) and apoptotic index(AI) analysis among cell kinetic parameters were used. As a result of PARP inhibitor application, there was a significant decrease in CI, MI and LI and a significant increase in AI for all the experimental groups. After application of PARP inhibitor in combination with nab‑paclitaxel, the CI values were decreased for both cell lines, and the differences between the control and all the experimental groups were statistically significant (P<0.01) for all applications. PARP inhibitor, alone or in combination with nab‑paclitaxel offers a promising treatment modality in different breast cancer subtypes.