IN VITRO CYTOPHYSIOLOGICAL ASPECTS INDUCED BY FOOD ADDITIVES FROM THE CATEGORY OF PRESERVATIVES ON SOME MAMMALIAN CELL MODELS

Abstract

This research is focused on cellular response to sodium metabisulphite (E223), sodium benzoate (E211), and sodium nitrite (E250) application on two normal mammalian cells, namely MCF-12A (ATCC CRL-3598) and Vero (ATCC CCL-81). The monitored parameters were cell viability (MTT assay), cell morphology (optical microscopy) and cell survival (clonogenic assay). The treatment was applied in doses of 12.5; 25; 50 and 100 µg/mL. According to all tests assessed, our results proved a dose-response relationship, the most sensibility presented the MCF-12A cell line.