In vitro culture of zygotic embryos and seeds of Caesalpinia ferrea Martius

Daniel Da Silva,Paulo De Tarso Barbosa Sampaio,Suely De Souza Costa,Angela Maria Imakawa

doi:10.1590/2236-8906-65/2018

Daniel Da Silva, Paulo De Tarso Barbosa Sampaio + Show 2 more

Open Access

https://doi.org/10.1590/2236-8906-65/2018

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Abstract

ABSTRACT The aim of this study was to evaluate the in vitro germination of zygotic embryos and seeds of Caesalpinia ferrea Martius and the morphogenetic responses of the explants to different concentrations of growth regulators. Seeds and zygotic embryos were inoculated in MS culture medium and kept in a growth room at a temperature of 25 ± 2 ºC for 16 hours of photoperiod for 30 days. The seeds had a higher in vitro germination rate than the explants from zygotic embryos. However, zygotic embryos in MS medium supplemented with 0.9 mg L-1 BAP had the highest percentage of regeneration (50%), number of shoots (3.25), buds (2.85) and leaves (3.15), multiplication rate (27.75), and length of shoots (1.96 cm). The in vitro culture of zygotic embryos and seeds made possible the multiplication of a higher number of healthy seedlings. Thus, it can be used as an alternative technique for the propagation of this species.

Highlights

Caesalpinia ferrea Mart. (Brazilian ironwood, “pau-ferro” or “jucá”) is a Brazilian tree species of economic potential and pharmaceutical interest due to its active principle called “Pau-ferrol”, extracted from the stems of juvenile plants and used against the human topoisomerase II, which inhibits the growth of cells through the induction of apoptosis, becoming an important tool in the treatment of human leukemia HL60 (Nozaki et al 2007; Ohira et al 2013)
In vitro seed germination resulted in a higher number of shoots (1.93), buds (1.33) and roots (3.40), sprout length (1.82 cm), and multiplication rate of explants four times higher than the explants from zygotic embryos of C. ferrea
The highest rates of regeneration and multiplication of C. ferrea zygotic embryos were obtained in MS culture medium supplemented with 0.9 mg L-1 BAP

Summary

Introduction

Caesalpinia ferrea Mart. (Brazilian ironwood, “pau-ferro” or “jucá”) is a Brazilian tree species of economic potential and pharmaceutical interest due to its active principle called “Pau-ferrol”, extracted from the stems of juvenile plants and used against the human topoisomerase II, which inhibits the growth of cells through the induction of apoptosis, becoming an important tool in the treatment of human leukemia HL60 (Nozaki et al 2007; Ohira et al 2013).The propagation of C. ferrea is viable by seeds (Scalon et al 2011) and by seedlings (Lima et al 2008; Lenhard et al 2010). In vitro propagation is a technique used in plant biotechnology that explores the totipotency of the nature of plant cells, which makes possible the reproduction of superior genotypes in vitro (Gatti et al 2016) through small fragments of living tissue (explants) isolated from an organism and grown in a nutritious culture medium. Such advances have increased the prospects of genetic manipulation. Universidade do Estado do Amazonas, Escola Superior de Tecnologia, Laboratório de Cultura de Tecidos Vegetais, Av. Darcy Vargas

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