Abstract
The growth of Toxoplasma gondii (RH strain) was compared in HEp2, VERO, MRC-5 and African green monkey primary kidney (AGMPK) cell lines. Optimal growth conditions with regard to parasite yield, purity and antigen secretion (assessed by immunoelectrophoresis) occurred when AGMPK were the host cells. The kinetics of antigen release from infected AGMPK cultures were studied, and although antigen recognized by ELISA was detectable after l h of culture, supernatants were only able to induce antigen-specific mitogenesis after 60 h culture, suggesting that certain components of secreted antigen may be functionally distinct. Continuous culture of T. gondii in AGMPK cells did not result in a change in virulence of the parasite. Toxoplasma from this cell line could also be used in the dye test for serodiagnosis, with no significant difference between culture- or mouse-derived parasites ( P > 0.05).
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