Abstract
The goal of this paper is to demonstrate in vitro techniques of culturing Entomopathogenic nematode and the diet on which EPN survive and flourish. The in vitro production of nematodes is essential for the commercial use of EPN. The competence of a range of media to promote growth and development of the nematodes has been evaluated. The diet consists of a base diet plus a nutritive supplement. Nematode yield is a crucial factor for successful large-scale viable production of entomopathogenic nematode. Few attempts at in vitro culture of EPNs have been undertaken. The various methods used to commence cultures are described. The diet named Kabuli gram medium constitute Nutrient broth (0.88g), yeast extract (0.32g), Kabuli gram (16g), soybean oil (10.40ml), and distilled water (60ml) proved effective, very nutritious and appropriate for nematode culture, with this diet EPN flourish in good quantity. In vitro culturing requires more attention, nominal temperature. A small negligence will destroy the colonies of EPN in no time. However, quality and quantity of in vitro produced Entomopathogenic nematode vary considerably, depending on diet and temperature. The EPN cultured, were used for the biocontrol of Helicoverpa armigera in further study on the crops of Pisum sativum and Cicer arietenum. The production and culture of EPN had help in the biocontrol. EPN used in present and further study were Steinernema masoodi and Oscheius nadarajni.
Highlights
In one way we can say that these nematodes serve as a vector for the bacteria to kill the pest rendering to their control
Entomopathogenic nematodes (EPN) of genus Steinernema, Heterorhabditis and Oscheius are symbiotically associated with bacteria of genus Xenorhabdus and Photorhabdus respectively
In a general sense, our results indicate diets can be optimized for improvement in vitro EPN production efficiency
Summary
1. Introduction having separate sexes and follow symbiotic relationship with bacteria, e.g. Xenorhabdus or Photorhabdus. Introduction having separate sexes and follow symbiotic relationship with bacteria, e.g. Xenorhabdus or Photorhabdus They spend life in soil as well as in insects. EPN of genus Steinernema, Heterorhabditis and Oscheius are symbiotically associated with bacteria of genus Xenorhabdus and Photorhabdus respectively. These are lethal obligatory parasites; yet pose no threat to plants. In a general sense, our results indicate diets can be optimized for improvement in vitro EPN production efficiency. This is the first report of an EPN diet.
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