Abstract
LAPAGE1 working with Haemonchus contortus reported in 1933 that: “Attempts have been made to cultivate the parasitic larval stages, a method having been found by which the free-living infective third stages may be exsheathed and sterilized, so that the first parasitic stages may be obtained and studied further in sterile media of known composition. Although these first parasitic stages have been studied in hanging drops in more than 200 media designed to imitate environmental factors to which parasites are presumably exposed in the body of the host no success has been obtained”.
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