In vitro correlation studies of antidiabetic, antioxidant activity and HPLC-ESI-MS/MS analysis of marine seaweeds from Gulf of Mannar

Abstract

Marine seaweeds are known to exhibit potential biological activity, which possess a variety of biological functions. The important pharmacological aspect of using marine polyphenols as a strong natural antioxidant has been suggested to play a vital role in the alleviation of diabetes mellitus. The present study aims to investigate the antioxidant potential of four green marine seaweeds namely Chaetomorpha crassa, Caulerpa racemosa, Caulerpa verticillata and Caulerpa scalpelliformis in the management of Type 2 diabetes mellitus. In vitro evaluation of the antioxidant activity was assessed by employing ABTS, Phosphomolybdenum, and metal chelation activity methods. The results showed that among four marine seaweeds, the ethanolic extracts of C. verticillata have recorded the highest total flavonoid content (66.19 ± 0.002 mg QE/g) and antioxidant activity in ABTS (96.95 ± 0.411 %), Phosphomolybdenum assay (323.28±0.006μM), and Metal chelation activity (71.92 ± 0.48%). The IC50 values of C verticillata were found to be 0.42 mg/ml for ABTS and 0.78 mg/ml for Metal chelation activity respectively. Also, ethanolic extracts of C verticillata showed highly significant inhibition of α-amylase (70.64 ± 0.39% with IC50 of 0.420 mg/ml) and α-Glucosidase (64.07 ± 0.33% with IC50 of 0.068 mg/ml) in a dose-dependent manner. The resultant principal component analysis had showed the effective association between the antioxidant and antidiabetic potential of the marine seaweeds. It is clear, that C. verticillata could be a good choice to reduce the hyperglycemic condition by inhibiting the carbohydrate hydrolyzing enzymes and maintain tolerable oxidative stress.