Abstract
In this talk, the characteristic motion associated with a large transmembrane protein (5HT3A∗) in a Double Cushion biomimetic assembly, is an assembly that is passivated with a Bovine Serum Albumin layer along with PEG(poly-ethylene glycol) as the cushion will be presented. These results will be compared to receptors in both planar POPC assemblies and Single cushion assemblies. The main conclusion of this work is that while FRAP recoveries (percent bulk mobility) of these large membrane receptors are quite low in these systems, Single Particle Tracking (SPT) has revealed reasonably large mobile fractions when viewed at much higher spatial resolution and slower time scales. This is especially true for receptors in the ‘Double cushion’ assemblies. These assemblies display very low FRAP recoveries, but SPT clearly reveal that nearly all receptors are actually quite mobile but are spatially confined to very small corrals. The high mobility of receptors in these corralling domains demonstrates that the strong attractive interactions between the transmembrane protein and solid support have been virtually eliminated and therefore it is reasonable to believe that these receptors will remain active.
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