In vitro conservation of Poincianella pyramidalis (Tul.) L.P. Queiroz under minimal growth conditions

Cristina Ferreira Nepomuceno,Tecla Dos Santos Silva,Taliane Leila Soares,José Raniere Ferreira De Santana

doi:10.1590/1413-7054201943014519

Cristina Ferreira Nepomuceno, Tecla Dos Santos Silva + Show 2 more

Open Access

https://doi.org/10.1590/1413-7054201943014519

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Abstract

ABSTRACT Poincianella pyramidalis (Tul.) L.P. Queiroz, is an arboreal species endemic to Brazil’s Caatinga biome and an important source of lumber and also of medicinal substances. This study aimed to evaluate the effect of the osmotic agents sucrose, sorbitol and mannitol and the growth retardant paclobutrazol (PBZ) on the induction of slow growth in vitro of P. pyramidalis, seeking to establish alternative techniques for ex situ conservation of the species. In the first experiment, plantlets with seven days of age were inoculated on Woody Plant Medium (WPM) supplemented with four concentrations of sucrose (87.64, 131.46, 175.28 or 219.10 mM) combined with 0.0 or 87.64 mM sorbitol or mannitol. In the second experiment, these plantlets were inoculated on WPM medium with 0.0, 1.5, 3.0, 4.5 or 6.0 µM PBZ and supplemented with 87.64 mM sucrose. For both experiments, the survival percentage of the plants was evaluated every 60 days, and at the end of 240 days, the following parameters were recorded: number of green leaves and senescent leaves; length of the aerial part and longest root; and dry mass of the aerial part and roots. The best results to maintain the quality of the P. pyramidalis plants conserved in vitro were obtained on medium supplemented with 219.10 mM sucrose for up to 240 days without subculture. With respect to PBZ, the concentration of 6.0 μM can be recommended for in vitro conservation of P. pyramidalis for 240 days without the need of subculturing.

Highlights

After 240 days, the highest survival rates of the catingueira plants were obtained on the medium only supplemented with sucrose: M3 with 77.78% (Table 2; Figure 2a), M4 with 72.78%, M1 with 68.05% and M2 with 67.46% (Table 2)
Studies performed with other species have produced divergent results regarding survival rates of plants conserved in vitro and submitted to slow growth induced by osmotic agents
El-Bahr et al (2016), investigating three osmotic substances for in vitro conservation to two date palm cultivars (Phoenix dactylifera L.) under slow growth observed that the culture medium containing only sucrose promoted the highest survival of the plants of the Sakkoty cultivar, while for the Bartamoda cultivar the highest survival percentages were obtained with the medium supplemented with 219.57 mM or 329.35 mM of mannitol and 109.78 m mM of sorbitol

Summary

Introduction

Is a mediumsized arboreal species belonging to the family Fabaceae (Leguminosae), endemic to Brazil’s semiarid northeastern region, with wide distribution in the Caatinga biome (Carvalho, 2014; Chaves et al, 2016; Souza et al, 2018). It is popularly known as ‘catingueira’, ‘pau-de-rato’, ‘catingueira-das-folhas-largas’, ‘mussitaiba’ and ‘paude-porco’ (Leite; Machado, 2009; Gomes-Copeland et al, 2017). Other biological and environmental factors, such as short annual production cycle of seeds, irregularity and uneven distribution of rainfall in the semiarid region from one year to the and seed dormancy (Alves et al, 2007), can interfere in obtaining catingueira seeds with the desired genetic and physiological qualities To overcome these limitations, in vitro methods are an important alternative to complement the conservation and prevent the genetic erosion of the species

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