Abstract
Cowpea chlorotic mottle virus (CCMV) RNA was translated in a reticulocyte lysate cell-free system, in presence of poly (A) +-RNA from developing cotyledons of garden pea ( Pisum sativum L.). In the subsaturating range of mRNA concentrations (⩽ 0.4 μg), increasing amounts of poly (A) +-RNA suppressed translation of CCMV RNAs coding for viral polypeptides of 102 000 and 38 000 M r, without having much effect on the translation of CCMV RNA coding for 20 000 and 17 000 M r. At higher mRNA concentrations, up to 1.4 μg, the ‘read out’ of all CCMV RNAs was arrested and this effect was not due to CCMV RNAs competing among themselves. Competition between viral mRNAs was however observed at supersaturating levels of mRNA concentration in favour of translation of low mol. wt. CCMV RNAs.
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