In Vitro Comparison of the Cytotoxicity and Water Sorption of Two Different Denture Base Systems

Abstract

Denture base resins have the potential to cause cytotoxicity in vivo, and the mechanical properties of resins are affected by water sorption. There is a correlation between residual monomer and water sorption. Thus, the purpose of this study was to evaluate water sorption and cytotoxicity of light-activated urethane dimethacrylate (UDMA) denture base resin compared to a conventional heat-activated polymethyl methacrylate (PMMA) resin. Two denture base resins, heat-activated PMMA (Meliodent) and light-activated UDMA (Eclipse), were used in this study. Cytotoxicity (5 × 1 mm(2) ) and water sorption (1 × 1 mm(2) ) specimens were made following the manufacturers' instructions (n = 10). Cytotoxicity tests of denture base resins were performed according to ISO10993-5:1999, and water sorption was evaluated according to ISO 1567:1997. ANOVA tests were employed for evaluating data (α = 0.05). There was no cytotoxic effect in either the PMMA or UDMA group. In addition, contrary to short-term water storage, a significantly lower water sorption value was shown for UDMA resins compared to PMMA resins in both 3- and 6-month storage periods (p = 0.043 and p = 0.002, respectively). The tested denture base materials adhered to the ISO standards for both cytotoxicity and water sorption. The cytotoxicity of the light-activated UDMA resin tested was statistically similar to that of the heat-activated PMMA resin; however, the UDMA resin exhibited decreased water sorption in long-term water storage.