Abstract
Soilless culture as in vitro culture could provide important sources of genetic variability useful for new cultivar selection. The objective of our study was to establish the culture conditions for in vitro micropropagation of some grapevine cultivars and to identify if the somaclonal variations occur during in vitro culture. The following cultivars were studied: 'Feteasca Regala', 'Muscat Ottonel', 'Brumariu' and 'Radames'. In vitro culture was induced by shoots explants. Shoots explants regenerated plants on all media studied, but multiplication is generally low and it was obtained especially on MS media supplementd with BA and NAA. The in vitro plantlets were analysed by mean of RAPD markers in order to identify the somaclonal variations. The best RAPD patterns have been obtained with OPE-3, OPO-4, OPO-7 and OPO-8 primers. These markers showed differences between cultivars but the polymorphism of in vitro plantlets was not observed. Somaclonal variation could not be identified by these RAPD markers. In vitro multiplication was in fact a clonal propagation.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
