Abstract
The present investigation has been concentrated on the establishment of an efficacious method for <i>in vitro</i> regeneration of a <i>Musa</i> cultivar Gopi of Tripura. Focus has been greatly anchored to the sterilization procedure for assuring successful aseptic culture establishment of this cultivar. The culture protocol for shoot induction and proliferation comprised of four different treatments in basal MS medium fortified with 6-Benzylaminopurine of varying concentrations. The optimum response was encountered in the treatment of BAP in MS medium at concentration of 8mg l<sup>-1</sup> for shoot bud induction and multiplication. Subsequent shoot growth and multiplication was achieved through repeated subcultures in media containing lower concentration (4 mg l<sup>-1</sup>). Root initiation was stimulated and brought about by treatments with two different concentrations of Indole butyric acid. The best rooting was manifested in treatment with IBA at concentration of 2mg l<sup>-1</sup>. The present study is an approach towards successful establishment of a simple and rapid clonal propagation of a potent Musa cultivar Gopi having traditional and commercial value from this region of Tripura, North-east India.
Highlights
Banana is one of the chief fruit crops for global food security and economy ranking fourth in the most crucial food commodities worldwide in terms of gross production [1,2,3]
In vitro morphogenic responses and adventitious shoot bud proliferation were investigated with different experimental sets of media supplemented with different concentrations of cytokinin BAP (2mg l-1, 4mg l-1, 6 mg l-1 and 8mg l-1)
After developing healthy elongated shoots in culture, they were transferred to MS medium supplemented with different concentrations of IBA viz. 2mg l-1, 4 mg l-1
Summary
Banana is one of the chief fruit crops for global food security and economy ranking fourth in the most crucial food commodities worldwide in terms of gross production [1,2,3]. Successful establishment of culture through multiple shoot formation was achieved by regular subculturing at 28 and 42 days interval on freshly prepared same medium. After developing healthy elongated shoots in culture, they were transferred to MS medium supplemented with different concentrations of IBA viz.
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