In Vitro Clonal Propagation of Korarima (Aframomum Corrorima (Braun) P.C.M. Jansen) on Improved Varieties of Bonga and Benchmajji

Abstract

Korarima (Aframomum corrorima (Braun) P.C.M. Jansen) is a diploid, 2n = 48, monocious, perennial and aromatic herb species under family Zingiberaceae. It is a native plant used as a spice in regular Ethiopian dishes and locally used in traditional medicine. However, commercial exploitation of this crop is hampered due to poor rate of seed germination and slow multiplication rate of the rhizomes along with other agronomic predicaments. Thus, the present study was aimed at in vitro micro propagations of two Aframomum corrorima cultivars of Bonga1 and Benchmajji1. Based on the results, better response was observed in rhizome bud explants with the highest mean number of shoots per explants, 4.33±0.33 and 4.67±0.33 for cv. Bonga1 and Benchmajji1 respectively, whereas 3.33±0.33 and 3.00±0.00 for cv. Bonga1 and Benchmajji1 respectively on the MS medium supplemented with BA 2.0mg/l and KN 0.5 mg/l. For multiplication, the highest mean number of shoots per explants (4.67±0.33) was obtained from the MS medium supplemented with combination of 3.0mg/l BA and 0.5 mg/l KN for cv. Bonga1, and the highest mean number of shoots per explants (4.00±0.00) was obtained from the MS medium supplemented with 2.0mg/l BA from shoot tip explants. For rooting induction, the highest mean number of roots per explants (15.00±1.15) was obtained in the MS supplemented with 1.0mg/l NAA for cv. Bonga1, and the highest mean number of roots per explants (17.33±0.88) was observed in the MS supplemented with 1.5mg/l IBA and 2.0mg/l for genotype cv. Benchmajji1, but the highest root length was obtained in ex vitro rooting with sand and vermi-compost at ratio of 2:1 with 1mg/l IBA three times per a week. Keywords: In vitro clonal propagation, Shoot multiplication, Rooting induction and Ex-vitro DOI: 10.7176/JBAH/12-17-04 Publication date: September 30 th 2022