Abstract
Failure in airway regeneration after injury may result in profound consequences like a disease with bronchiolitis obliterans. Re-building of ciliated cells is a good marker for resumed airway activity. We decided to investigate whether M3E3C3,a fetal hamster stem equivalent cell line, is capable of in vitro ciliogenesis. Immunoreactivity and qRT-PCR have revealed that this cell line expresses a stem cell marker Sox2 and another marker, Clara cell secretory protein (CCSP) specific for the stem cells in the bronchiolar region. An embryonic stem cell factor Oct4 was negative.
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