Abstract
Chromosomes of the root vole, Microtus oeconomus, were studied in early passages of cultures of adult cells and the female and male idiograms were calculated from five cells of each sex. This species shows chromosomal polymorphism in nature, presumably caused by centric fission. One culture from a female animal, heterozygous for centric fission in one metacentric pair, was followed through 143 passages during a period of 21 months. Transformation from diploidy to heteroploidy took place after passages 40–50. The stemline remained in the diploid region throughout the experiment, the stemline number changing from 31 to 30. The chromosomes involved in the centric fission remained stable. Another series of examinations of a long-term culture, started from a frozen sample of passage 16 of the same culture, showed essentially similar chromosomal pattern, indicating that the chromosomal development of the permanent cell line was determined by the genotype of the original cells.
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