Abstract
Confluent cultured normal human skin fibroblasts from neonatal, adult and aged donors have been stimulated to respond to wounding of the cell sheet. The latent period prior to initial migration of cells from the leading edge of the monolayer is correlated with in vitro population doubling level and in vivo donor age. Time-lapse photography of areas along the edge of the cell sheet reveals a specific pattern of migration by which the cells reestablish a confluent monolayer.
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