In Vitro Characterization of Poly-Glycolyc Lactic-Co Acid (PLGA) –Collagen Based on Red Snapper Fish Scales (Lutjanus Sp.) Coating Chitosan as Duramater Artificial Candidate

Abstract

Head trauma is the third cause of deaths with a high rank, causing severe head injury for 25.5%-54.9%. This study has been conducted by making a replacement layer of the brain (dura) to overcome the dural defect's impact by utilizing waste fish scales red snapper (Lutjanus Sp.). Synthesis brain membranes lining processed by casting method with each various chitosan coating concentrations of 1%, 1.5%, and 2%, then dried using vacuum dry. The samples were then characterized by tensile tests, FTIR, SEM, and MTT Assay. FTIR test results showed that red snipper scales could produce collagen powder at amide A group with stretching of –NH functional group, amide B group, has stretching of CH2 asymmetry, amide I area, amide II, and amide III area, which show –NH bonding. Tensile test results showed that the combination of PLGA-Collagen Chitosan Coating 2% produced the highest tensile strength is 4.8 MPa, which met human dura mater strength standards. MTT Assay results showed that the dural membrane produced no toxic seen from living cells reached 98.32%. Poly - Glycolic Lactic - Co Acid (PLGA) - collagen coating chitosan-based on red snapper fish scales (Lutjanus Sp.) composites has potency dura mater, artificial candidate, due to the chemistry, biological, and physical characteristics.