Abstract
To better define the role of the chicken haemopoietic microenvironment in supporting haemopoiesis, a continuous cell line was generated by RSV transformation of avian spleen stromal cells (SSL-1). Super-natants from this line were found to contain haemopoietic growth factor activity as measured by the ability to induce proliferation and differentiation of precursor cells present in embryonic and post-hatched haemo-poietic tissues. Comparison of cultures grown in the presence of cMGF and SSL-1 conditioned media (CM) revealed that both cytokine sources induced similar types of cell populations. Both sources supported the proliferation of predominantly macrophage-like cells based on colony morphology, differential staining, non-specific esterase staining, and phagocytosis activity. Interestingly, SSL-1 does not express any message for cMGF, nor does it secrete any IL-2 or Interferon activities suggesting that the growth factor activities seen in SSL-1 are novel.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
