Abstract
Syngeneic mouse models of breast cancer offer the researcher an important tool with which to investigate the molecular mechanisms of cancer. These models generally arise through the isolation of breast cancer cells from spontaneous breast tumours in mice. As the cells have originated from a spontaneous tumour, they are immunologically and genetically compatible to a more clinically relevant breast cancer model. The aim of this study was to characterise newly isolated Spontaneous Mammary Tumour (SMT1) cells and its matched lung metastatic cells (SMT1L) from a spontaneous mammary tumour that arose in a 2 year old female BALB/c mouse.
Highlights
Syngeneic mouse models of breast cancer offer the researcher an important tool with which to investigate the molecular mechanisms of cancer
The aim of this study was to characterise newly isolated Spontaneous Mammary Tumour (SMT1) cells and its matched lung metastatic cells (SMT1L) from a spontaneous mammary tumour that arose in a 2 year old female BALB/c mouse
Western blot analysis using cell lysates from SMT cells was used to confirm that cells were of epithelial origin, to characterise the cells for biomarkers of breast cancer subtypes: oestrogen receptor, progesterone receptor, ErbB2 (Her2) and Epidermal Growth Factor Receptor (EGFR) expression and to verify expression of the Insulin-like Growth Factor (IGF) pathway (the IGF receptor (IGF-IR) and Insulin Receptor (IR))
Summary
Syngeneic mouse models of breast cancer offer the researcher an important tool with which to investigate the molecular mechanisms of cancer. These models generally arise through the isolation of breast cancer cells from spontaneous breast tumours in mice. As the cells have originated from a spontaneous tumour, they are immunologically and genetically compatible to a more clinically relevant breast cancer model. The aim of this study was to characterise newly isolated Spontaneous Mammary Tumour (SMT1) cells and its matched lung metastatic cells (SMT1L) from a spontaneous mammary tumour that arose in a 2 year old female BALB/c mouse
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