Abstract
The present study was undertaken to evaluate the impact of Biofield Energy Treated test formulation using multiple cell-lines. The test formulation and cell media (Med) was divided into two parts; one part was untreated (UT) and other part received Biofield Energy Treatment remotely by a renowned Biofield Energy Healer, Krista Joanne Callas, USA and labeled as Biofield Energy Treated (BT) test item (TI)/Med. Based on cell viability, test formulation was found safe. Cytoprotective action of test formulation showed significant restoration of cell viability by 89.9% and 106.4% in human cardiac fibroblasts cells (HCF) cells, while improved restoration of cell viability by 77.3% and 69% in HepG2 cells compared to untreated. Cellular restoration in A549 cells was also improved by 141.2% and 157.1% compared to untreated. ALP activity was significantly increased by 118.7% and 140.7% in UT-Med + BT-TI and BT-Med + UT-TI, respectively at 0.1 µg/mL than untreated. Percent cellular protection of HCF (heart) cells (decreased of LDH activity) was significantly increased by 89.9% and 106.4% in UT-Med + BT-TI and BT-Med + BT-TI, respectively than untreated. HepG2 cells protection (decreased ALT activity) was increased by 59.8% in BT-Med + BT-TI than untreated. Superoxide dismutase (SOD) level was increased by 22.8% in BT-Med + BT-TI than untreated. Serotonin level was significantly increased by 361.7% and 197.6% in BT-Med + UT-TI and BT-Med + BT-TI, respectively than untreated in human neuroblastoma cells (SH-SY5Y). However, relative quantification (RQ) of vitamin D receptor (VDR) was significantly increased by 116.5%, 214.7%, and 241.5% in UT-Med + BT-TI, BT-Med + UT-TI, and BT-Med + BT-TI, respectively than untreated in MG-63 cells. Overall, data showed a significant improvement of organ-specific functional enzyme biomarkers. Thus, Biofield Energy Treated Test formulation (the Trivedi Effect®) would be useful for multiple organs health that can be beneficial against coronary artery disease, arrhythmias, congenital heart disease, cardiomyopathy, cirrhosis, liver cancer, hemochromatosis, asthma, chronic bronchitis, cystic fibrosis, osteoporosis, etc.
Highlights
Alternative system of medicine includes treatments different from mainstream therapeutic approach denoted as integrative or complementary medicine
In HepG2 cells restoration of cell viability was observed as 77.3% and 69% in Biofield Energy Treated (BT)-Med + UT-test item (TI) and Biofield Energy Treated medium (BT-Med) + Biofield Energy Treated formulation (BT-TI) groups, respectively as compared to untreated at 10 μg/mL
In A549 cells, cellular restoration was improved by 141.2% and 157.1% at 0.1 μg/mL in UT-Med + BT-TI and BT-Med + BT-TI groups, respectively as compared to untreated
Summary
Alternative system of medicine includes treatments different from mainstream therapeutic approach denoted as integrative or complementary medicine. Owing to the recent literature and availability of the database with respect to herbal drug formulation, a novel herbomineral test formulation was developed for overall functioning of multiple organs. This test formulation included Panax ginseng extract, beta carotene, calcium chloride, magnesium gluconate, zinc chloride, sodium selenate, ferrous sulfate, vitamin B12, vitamin D3, ascorbic acid, and vitamin B6. A potent immunomodulator was reported to have improved wellness and thinking, memory, concentration, physical stamina, work efficiency, preventing muscle damage, Alzheimer's disease, athletic endurance, improve mental and cognitive health [9, 10] This formulation was tested using standard organ functioning specific cell line based assays for different biological activities. The test formulation and the cell based specific media was treated with the one of the complementary medicine i.e. Biofield Energy (The Trivedi Effect®-Consciousness Energy Healing) by a renowned Biofield Energy Healer
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