In vitro cell system for studying molecular mechanisms of action associated with low intensity focused ultrasound

Abstract

Low intensity focused ultrasound (LIFU) is now being considered as a noninvasive brain therapy for clinical applications. We maintain that LIFU can efficiently deliver energy from outside the skull to target specific brain regions, effecting localized neuromodulation. However, the underlying molecular mechanisms that drive this LIFU-induced neuromodulation are not well-defined due, in part, to our lack of understanding of how particular sets of LIFU delivery parameters affect the outcome. To efficiently conduct multiple sweeps of different parameters and determine their effects, we have developed an <i>in-vitro</i> system to study the effects of LIFU on different types of cells grown in culture. Presently, we are evaluating how LIFU affects the ionic flux that may underlie neuronal excitation and inhibition observed <i>in-vivo</i>. The results of our <i>in-vitro</i> studies will provide a rationale for selection of optimal LIFU parameter to be used in subsequent <i>in-vivo</i> applications. Thus, a prototype ultrasound cell assay system has been developed to conduct these studies, and is described in this work.