Abstract
Testes removed from 30-hr old pupae of Drosophila hydei were placed in vitro for various lengths of time. The cell contents of these testes were characterized at the time of explanation and at 2 days, 5 days and 7 days in vitro. Studies of the differentiation of the testes for comparable lengths of time were simultaneously carried out in vivo. Comparisons of the in vivo and the in vitro results indicate that while the testes in vitro do not undergo any morphological development whatsoever, the differentiation of the various cells therein appears to be the same as that observed in testes which have developed in vivo for the same lengths of time. This finding indicates that not only can the testes of Drosophila hydei be maintained in vitro for reasonably long periods of time but, also suggests that the cellular differentiation which occurs during the in vitro culture period may parallel that in vivo.
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