Abstract
Callus induction was tried with leaf explants (third leaf from top of canopy) harvested from in situ control and supplementary UV-B irradiated (UV-B = 2 hours daily @ 12.2 kJ m-2 d-1; ambient = 10 kJ m-2 d-1) three varieties of green gram viz. CO-8, NVL-585 and VAMBAN-2 to study their viability for germplam conservation. Callus induction occurred both in control and UV-B stressed NVL-585 leaf explants. VAMBAN-2 both in unstressed and UV-B stressed conditions did not initiate callus. Only control leaf explants from CO-8 proliferated callus. Callus of UV-B irradiated NVL-585 weighed less (51.28 %) than control. Parenchyma cells were smaller in callus inducted from in situ UV-B exposed NVL-585 leaf explants. The leaf explants from UV-B stressed NVL-585 varieties of green gram responded to in vitro callus proliferation making them fit for germplasm conservation for cultivating in UV-B elevated environment.Int J ApplSci Biotechnol, Vol 4(1): 82-91
Highlights
Ozone depletion is facilitated by increased stratospheric particles as provided by stratospheric clouds in the polar regions and globally by volcanic eruptions
In vitro callus induction In leaf explants proliferation of callus occurred only in two green gram varieties out of the three varieties taken for study (Table 1; Plate 2, 3)
Callus induction was observed in control leaf explants of CO-8 and both in control leaf explants as well as in leaf explants harvested from in situ supplementary UV-B irradiated NVL-585 crops
Summary
Ozone depletion is facilitated by increased stratospheric particles as provided by stratospheric clouds in the polar regions and globally by volcanic eruptions. This paper can be downloaded online at http://ijasbt.org&http://nepjol.info/index.php/IJASBT
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