Abstract
Guava (Psidium guajava L.) is an important tropical fruit rich in vitamins and vitamin precursors, minerals, organic acids, and pectins. Seventy-nine phytochemicals provide guava with many unique properties and actions including anti-microbial, astringent, bactericidal, cicatrizant, emmenagogue, hypoglycemic, laxative, nutritive, and spasmolytic. The research goal was to develop an efficient in vitro callus regeneration protocol for guava. This study evaluated specific culture media and plant growth regulators for their influences on callus initiation and production. Different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), or 6-benzyladenine (BA) were added to Murashige and Skoog (MS, 1962) and woody plant media (WPM, Lloyd and McCown, 1981). MS culture medium strength and guava cultivars were also investigated. 2,4-D at 1.6 mg/L stimulated the most callus production for the test cultivar, 'Beaumont', regardless of the basal culture medium. The amount of callus produced per explant was significantly different among the seven test cultivars. Higher concentration (2x) of MS salts stimulated callus production.
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