Abstract
The success of genetic transformation depends on the efficiency and rehability of in vitro shoot regeneration. This research was pursued to investigate how different plant growth regulators influence alfalfa callus initiation and development, thus to establish a foundation for further development of an efficient shoot organogenesis protocol for the genetic transformation system. BA, zeatin, and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) were evaluated for callus initiation and development. BA at 1 or 5 mg·liter–1, or zeatin at 5 mg·liter–1 promoted callus regeneration and further development toward shoot organogenesis. However, 2,4,5-T at 1 mg·liter–1 enhanced only callus production. These results can and will be used for further development of a shoot regeneration protocol to assist alfalfa genetic transformation.
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