In Vitro Callus Induction from Adult Tissues of Japanese Flowering Cherry Trees and Two Cherry Rootstocks

Dragana M Skočajić,Mirjana T Šijačić-Nikolić,Mihailo N Grbić,Marina Ž Nonić,Milica M Fotirić Akšić,Matilda Š Đukić,Marija M Nešić

doi:10.15835/nbha45210899

Dragana M Skočajić, Mirjana T Šijačić-Nikolić + Show 5 more

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https://doi.org/10.15835/nbha45210899

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Abstract

Several in vitro biotechnological techniques have been developed, all of which require a reliable protocol to produce a responsive callus mass. One of these techniques is callus fusion in vitro, which is reliable for the early detection of (in)-compatibility of scions and rootstocks. In this paper, the possibility to obtain friable callus tissues was explored by callus induction of adult tissues of Japanese flowering cherry trees from the group Sato zakura (Prunus serrulata ‘Amanogawa’, ‘Kanzan’ and ‘Kiku-shidare-zakura’) and two domestic cherry rootstocks – Prunus avium and Prunus ‘Colt’. The explants used in the research were: leaf petiole, leaf base with a part of a petiole, part of lamina with a midvein and a stem with an axillary bud. Among three plant growth media (MS, SH and WP) that were used in this study, the MS proved to be the most favourable for the majority of taxa during the callus induction process. For the sweet cherry tree and the cultivars ‘Kanzan’ and ‘Colt’, the SH plant growth medium was also acceptable. The best results in callogenesis were obtained for the majority of taxons with auxin at the concentration 2 mgL-1 NAA and cytokinin BAP 0.5 mgL-1. It is also possible to use 2.4-D at the same concentration as a substitute for the genotypes Prunus avium, Prunus ‘Colt’ and Prunus serrulata ‘Kanzan’, whereas IBA proved to be an inappropriate auxin for callus induction. The protocol described herein is proved to be efficient callus induction in a range of taxa of genus Prunus.

Highlights

Flowering cherries have been cultivated for more than 1000 years (Kato et al, 1999) and the economic and commercial importance is based on their attractive appearance, extensive vegetation, unpretentious growth conditions and high adaptation abilities (Kalinina and Brown, 2007)
The aim of this study was to establish an efficient protocol for callus induction from adult tissue so that the results obtained can be further used for the compatibility testing by callus fusion
Other types of explants on the SH medium induce the callus in percentages lower than 50%

Summary

Introduction

Cerasus; Rosaceae) have been cultivated for more than 1000 years (Kato et al, 1999) and the economic and commercial importance is based on their attractive appearance, extensive vegetation, unpretentious growth conditions and high adaptation abilities (Kalinina and Brown, 2007). Published reports of tissue culture experiments of ornamental species have been limited for only a few of them: Prunus lannesiana Wils. (Ishikura, 1994; Akita et al, 2006), eight ornamental cherries (Hokanson and Pooler, 2000), Prunus incisa ‘February Pink’ (Cheong and Pooler, 2004) and P. serrulata ‘Kanzan’ (Duta et al, 2007; Kalinina and Brown, 2007). Received in revised form: 02 Aug 2017.

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