Abstract
Abstract Aromatic rice (Oryza sativa L.) cultivars are strong aromatic rice cultivars which can thrive well in rice fields prone to flood, drought and other soil constraints. The present investigation was undertaken to determine a suitable media compositions for callus induction and regeneration using immature embryo of six aromatic grown rice cultivars of Bangladesh, namely, Chinigura, Kalijira, Radhuni Pagal, Modhumala, Kataribog and Mohonbhog. For callus induction different concentrations and combinations of 2, 4-Dichlorophenoxyaceticacid (2, 4-D) along with NAA were evaluated. Maximum callus induction (97.22%) was observed in Kalijira when 2 mg/L of 2, 4-D and 0.5 mg/l NAA was used and less Modhumala (66.67%) and remaining cultivars showed moderate. For regeneration initially different concentrations and combinations of 6-BenzylAminoPurine (BAP) and Indole-3-Acetic Acid (IBA) were tested. Maximum regeneration frequency (91.67%) was observed Kalijira when the optimum concentrations and combinations of 0.5 mg/l of BAP + 0.1 of mg/l IBA were used. Presently optimized regeneration method holds promise for facilitating the deployment of agronomical important trait through genetic transformation for the improvement of this important food crops.DOI: http://dx.doi.org/10.3126/ijasbt.v2i2.10313Int J Appl Sci Biotechnol, Vol. 2(2): 160-167
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: International Journal of Applied Sciences and Biotechnology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
