In-vitro Callus Induction and Regeneration of Brinjal (Solanum melongena L.) through Cotyledon

Abstract

Brinjal is one of the most popular, nutritional and vegetable crops in the world. It plays a vital role in the national economy as a cash crop. Tissue culture techniques used for in-vitro plant regeneration through cotyledon explants of eggplant (Solanum melongena L.) with different combinations of plant growth hormones BAP (4.44, 6.66, 8.88, 11.10 and 13.32µM) and IAA (0.57, 1.14 and 1.71µM) used for in-vitro regeneration of brinjal. The cotyledon explants used in this study, the highest callus induction found on BAP 8.88 µM and IAA 1.14 µM. The callus induction occurred after 15days from initiation, shoot induction occurred after 30 days from initiation and shoot elongation was carried out on the same medium, shoot elongation occurred after 45 days from initiation. MS hormone-free medium found best for root regeneration, the elongated shoots were selected and transferred to a test tube containing MS hormone-free rooting medium and the elongated shoots produce roots after 15 days. Then the rooted plantlets were transferred to poly-cup with a pre-sterilized mixture of coco peat for primary hardening under poly-tunnel for 10days. Subsequently, there generated plantlets acclimatized under the greenhouse. Then, hardened plants transferred to the open field for further development. This plant regeneration method can be useful for the production of the disease-free plant.