In- vitro biorelevant media and time simulation probiotic proliferation methodology to determine prebiotic potentials of flaxseed powder

Abstract

Dysbiosis is defined as disorganization of microflora homeostasis, leading to changes in metabolic activities and local distribution with major impacts on functional composition. Presently, gut microbiota modifications, with the objective to treat microbial dysbiosis, is well known treatment option preferred for conditions involving gut flora disturbances. It has been found that the most common approach to treat gut flora disturbance is to prescribe probiotic supplements. The objective of the present research was to develop simple methodology in order to determine the prebiotic potential of flaxseed powder using a novel in-vitro bio-relevant media and a time simulation proliferation approach for the marketed probiotic Bacillus coagulans SNZ 1969, (“Sporlac Sachets, Sanzyme Biologics Private Limited”). In addition to the desired result, the current study also yielded pertinent information on fermented flaxseed powder and helpful data regarding prescription guidelines for commercially available probiotic supplements. It has been observed that various factors like gastrointestinal pH, transit time, gastrointestinal fluid composition, presence of prebiotics, and fermentation play an important role in the proliferation and gastrointestinal colonization of orally ingested probiotics. The research findings justify the importance of synbiotic and postbiotics supplement approaches as the part of treatment and prescription requirements in gut flora microbial dysbiosis rather than probiotic supplements alone. Alternatively, patients could be guided for the concept that the consumption of prebiotic-containing foods have potential to enhance the ability of probiotics to restore the gut flora following a course of antibiotic therapy.