Abstract
The carbohydrate-hydrolyzing enzymes play a crucial role in increasing the phenolic content and nutritional properties of polysaccharides substrate, essential for cost-effective industrial applications. Also, improving the feed efficiency of poultry is essential to achieve significant economic benefits. The current study introduced a novel thermostable metagenome-derived xylanase named PersiXyn8 and investigated its synergistic effect with previously reported α-amylase (PersiAmy3) to enhance poultry feed utilization. The potential of the enzyme cocktail in the degradation of poultry feed was analyzed and showed 346.73 mg/g poultry feed reducing sugar after 72 h of hydrolysis. Next, the impact of solid-state fermentation on corn quality was investigated in the presence and absence of enzymes. The phenolic content increased from 36.60 mg/g GAE in control sample to 68.23 mg/g in the presence of enzymes. In addition, the enzyme-treated sample showed the highest reducing power OD 700 of 0.217 and the most potent radical scavenging activity against ABTS (40.36%) and DPPH (45.21%) radicals. Moreover, the protein and ash contents of the fermented corn increased by 4.88% and 6.46%, respectively. These results confirmed the potential of the carbohydrate-hydrolyzing enzymes cocktail as a low-cost treatment for improving the phenolic content, antioxidant activity, and nutritional values of corn for supplementation of corn-based poultry feed.
Highlights
The carbohydrate-hydrolyzing enzymes play a crucial role in increasing the phenolic content and nutritional properties of polysaccharides substrate, essential for cost-effective industrial applications
All chemicals including 3,5-Dinitrosalicylic acid (DNS), Bovine serum albumin (BSA), beechwood xylan, N a2CO3, avicel, β-glucan, xylan, filter paper, locust bean gum (LBG), carboxymethylcellulose (CMC), coomassie brilliant blue, ethanol, phosphoric acid, metal ions, ethylenediaminetetra acetic acid (EDTA), urea, phenylmethylsulfonyl fluoride (PMSF), sodium dodecyl sulfate (SDS), cetrimonium bromide (CTAB), Tween 20, Triton X-100, NaN3, HCl, NaOH, beta mercaptoethanol, methanol, acrylamide, bisacrylamide, temed, ammonium persulfate (APS), glycine, tris-base, 2,2’-azino-bis(3ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), potassium ferricyanide, trichloroacetic acid, ferric chloride, Folin and gallic acid were from Sigma-Aldrich
Carbohydrate- hydrolyzing enzymes can increase the hydrolysis of cellulose and hemicellulose and be used to produce high-value products
Summary
The carbohydrate-hydrolyzing enzymes play a crucial role in increasing the phenolic content and nutritional properties of polysaccharides substrate, essential for cost-effective industrial applications. The protein and ash contents of the fermented corn increased by 4.88% and 6.46%, respectively These results confirmed the potential of the carbohydrate-hydrolyzing enzymes cocktail as a low-cost treatment for improving the phenolic content, antioxidant activity, and nutritional values of corn for supplementation of corn-based poultry feed. The in-silico screening strategy and computationally assisted methodology were reported as a powerful method for the identification of several improved enzymes for industrial a pplications[21,22,23,24] This pipeline was used to identify the novel potential xylanases and amylases for effective degradation of raw materials[25,26]. The change in the phenolic content, antioxidant capacity, and nutritional value of the corn were investigated for its applicability in improving poultry feed nutrition and supplementation of cornbased diets
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
