Abstract
Novel prognostic biomarkers or therapeutic molecular targets for laryngeal squamous cell carcinoma (LSCC) are an urgent priority. We here sought to identify multiple novel LSCC-associated genes. Using high-density microarray expression profiling, we identified multiple genes that were significantly altered between human LSCCs and paired normal tissues. Potential oncogenic functions of one such gene, DCUN1D5, were further characterized in vitro. Our results demonstrated that DCUN1D5 was highly expressed in LSCCs. Overexpression of DCUN1D5 in vitro resulted in 2.7-fold increased cellular migration, 67.5% increased invasive capacity, and 2.6-fold increased proliferation. Endogenous DCUN1D5 expression was decreased in a time-dependent manner after genotoxic stress, and silencing of DCUN1D5 by siRNA decreased the number of cells in the S phase by 10.2% and increased apoptosis by 11.7%. Our data suggest that DCUN1D5 in vitro might have vital roles in DNA damage response, but further studies are warranted to assess its significance in vivo.
Highlights
Squamous cell carcinoma accounts for 90% of all malignancies of the larynx (Curado and Hashibe, 2009)
Endogenous DCUN1D5 expression was decreased in a time-dependent manner after genotoxic stress, and silencing of DCUN1D5 by siRNA decreased the number of cells in the S phase by 10.2% and increased apoptosis by 11.7%
With use of high-density microarray profiling to screen for gene expression patterns in laryngeal squamous cell carcinoma (LSCC), we found that DCUN1D5 (DCN1, defective in cullin neddylation 1, domain containing 5) was up-regulated in tumor samples of patients with LSCC who had a history of cigarette smoking
Summary
Squamous cell carcinoma accounts for 90% of all malignancies of the larynx (Curado and Hashibe, 2009). Endogenous DCUN1D5 expression was decreased in a time-dependent manner after genotoxic stress, and silencing of DCUN1D5 by siRNA decreased the number of cells in the S phase by 10.2% and increased apoptosis by 11.7%. Subcellular localization of DCUN1D5 Similar to the results of immunohistochemistry in squamous epithelial cancerous tissues (Figure 1c), subcellular localization analysis indicated that endogenous DCUN1D5 localized to the nucleus in Hep2 cells (Figure 1d) and HeLa cells.
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