Abstract
The in vitro biocontrol ability of Trichoderma harzianum on the phytopathogen Alternaria alternata improved in the presence of the growth regulators gibberellic acid (GA 3 ), or indolacetic acid (IAA) or benzylaminopurine (BAP) or foliar nutrient at concentrations similar or higher than those used at the field level. These plant hormones decreased the secretion of endopolygalacturonase (endo-PG) of A. alternata by approximately 20%, did not modify endochitinase (endo-CH) secretion of T. harzianum and did not alter germination of conidia or mycelia growth of any of these fungi. The presence of T. harzianum decreased endo-PGase secretion of A. alternata by about 50%. This inhibitory effect was independent of the presence of growth regulators. The level of secreted endo-PG of T. harzianum was not modified by the presence of A. alternata , but the presence of this phytopathogen in cultures of T. harzianum , increased both the growth of the biocontroller and its secretion of endo-CH.
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