Abstract
The present study probes into the biocompatibility testing of Phycobiliproteins (PBPs) fromGracilaria changii, in-vitro. The cell survivability and cytotoxicity of PBPs to cultured Human Cervix Carcinoma (Hep-2C) cells were tested and observed using MTT (3-(4, 5- Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay. For ten-fold dilutions, Phycobiliproteins (PBPs) promotes highest cell survivability at 10-4 dilution rate with 239.62% and highest cytotoxic effect, with 40.21%, at 10-1 dilution. The compositions of phycobiliproteins were determined using Ultraviolet (UV-Vis) spectrophotometer giving visible absorbance peaks at 498.5, 614 and 651 nm for the three PBPs composition: Phycoerythrin (PE), Phycocyanin (PC) and Allophycocyanin (APC), respectively. The effects of time duration in centrifugation process of Phycobiliproteins (PBPs) fromGracilaria changiiin 3, 5 and 7 minutes, were proven significant on the quantity of the extracted PBPs. The highest average concentration of 20.37 µg/ml for APC, 13.31 µg/ml for PC and 18.57 µg/ml for PE were obtained at the 5 minute-extraction process.
Highlights
The rising demand for natural instead of synthetic materials especially in biomedical applications where high biocompatibility and no adverse effects for the host organism are required (Mano et al 2007), has led to an outburst of scientific papers involved in the study of biobased materials
Due to high absorption coefficients and quantum yields of phycobiliproteins, absorption peaks obtained using UVVis spectrophotometer became visible at the reference wavelength of Phycoerythrin, Phycocyanin and Allophycocyanin
The results showed that there is a certain dilution rate that affects the behavior of Phycobiliprotein 1 concentration as was proven by(Wang et al 2012), in which they expressed that the cell demise reaction to Photodynamic treatment (PDT) of phycobiliprotein differs because of the physical properties of the photosensitizer utilized, the PDT measurements and the cell sort and inhibits the growth of Hep-2C cells, as was proven by
Summary
The rising demand for natural instead of synthetic materials especially in biomedical applications where high biocompatibility and no adverse effects for the host organism are required (Mano et al 2007), has led to an outburst of scientific papers involved in the study of biobased materials. In this view, the feasibility of using starting materials obtainable from organic sources has seen potential for such purpose. Phycobiliproteins (PBPs), consisting of phycoerythrin (PE), phycocyanin (PC) and allophycocyanin (APC), are the principal photoreceptors for photosynthesis in bluegreen and red as well as some other types of macro algae. PBPs are mainly used as fluorescent probes that are expensive after commercialization (Cai et al 2012); the effects of PBPs on anti-oxidation, anti- tumor, anti-virus, anti-inflammation, liver-protection, neuro-protection, UV protection, atherosclerosis and skin function activating have been demonstrated
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