In Vitro Biocompatibility and Antibacterial Property of a Novel Magnesium Phosphate Whisker

Abstract

The in vitro biocompatibility of a novel magnesium phosphate whisker (M-PW) was evaluated by real-time cellular analysis (RTCA) and Annexin-V/PI double marking methods, and its antibacterial property was evaluated by co-culture method. Results show that the in vitro biocompatibility of the MPW decreased with the increase of M-PW in the suspensions. It possessed excellent in vitro biocompatibility for the suspensions containing 500 μg/mL or lower amount of the M-PW. It had no toxic effect on osteoblast cells for the suspension with 50 and 200 μg/mL of M-PW respectively. The antibacterial efficacy of the suspensions increased with the increasing amount of MPW. The antibacterial efficacy against Escherichia coli and Staphylococcus aureus achieved 96.84% and 99.93% respectively for the suspension with 500 μg/mL of M-PW, demonstrating that the novel phosphorous-magnesium whisker possesses excellent antibacterial property.