In vitro bioactivity and cytotoxicity of chemically treated glass fibers

Ângela Leão Andrade,Regina Maria De Marco Turchetti-Maia,Míriam Teresa Paz Lopes,Carlos Edmundo Salas,Rosana Zacarias Domingues

doi:10.1590/s1516-14392004000400019

Ângela Leão Andrade, Regina Maria De Marco Turchetti-Maia + Show 3 more

Open Access

https://doi.org/10.1590/s1516-14392004000400019

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Journal: Materials Research	Publication Date: Dec 1, 2004
Citations: 9	License type: cc-by

Affiliation: Universidade Federal de Minas Gerais

Abstract

Samples of a commercial glass fiber FM® (Fiber Max) were used to test the efficacy of a chemical sol-gel surface treatment to enhance their bioactivity. After treatment with tetraethoxysilane (TEOS), individual fiber samples were soaked into a simulated body fluid (SBF) solution, from which they were removed at intervals of 5 and 10 days. Micrographs obtained by scanning electron microscopy (SEM) analysis of samples chemically treated with TEOS revealed the formation of a hydroxyapatite (HA) coating layer after 5 days into SBF solution. Fourier transform infrared spectroscopic (FTIR) analyses confirmed that the coating layer has P-O vibration bands characteristic of HA. The in vitro cytotoxicity was evaluated using a direct contact test, minimum essential medium elution test (ISO 10993-5) and MTT assay. Fibers immersed in SBF and their extracts exhibited lower cytotoxicity than the controls not subjected to immersion, suggesting that SBF treatment improves the biocompatibility of the fiber.

Highlights

IntroductionBioactive glasses can develop a layer of hydroxyapatite (HA) on their surface when in contact with biological fluids or tissues[1,2]
A well recognized method to test the in vitro bioactivity of ceramic materials consists in their immersion in an aqueous solution Simulated Body Fluid (SBF) which simulates the properties of human plasma for a certain period, and verify the formation of the hydroxyapatite (HA) layer on the surface of the sample[3]
The purpose of this work was to assess the in vitro bioactivity and cytotoxicity of FM ® glass fibers treated with tetraethoxysilane (TEOS) and simulated body fluid (SBF)

Summary

Introduction

Bioactive glasses can develop a layer of hydroxyapatite (HA) on their surface when in contact with biological fluids or tissues[1,2]. It is important to note that commercial bioactive fibers are not available and for future purposes the use of fibrous materials was desirable since the presence of high porosity is one of the basic requirements for a good vascularization and subsequent growth of neo-bone. This evaluation is part of a process to validate the usefulness of the fiber in the biomedical therapy

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