Abstract
This work addresses the effect of sodium caseinate (SC) and β-lactoglobulin (β-Lg) glycosylated with galactose (Gal) via the Maillard reaction on the human intestinal microbiota, using small-scale in vitro batch cultures inoculated with human faeces. The glycation process was controlled to maximise formation of the corresponding Amadori compound, tagatosyl-lysine, a derivative of tagatose. Both glycoconjugates (SC:Gal and β-Lg:Gal) induced a significant increase in bifidobacteria, whilst Lactobacillus–Enterococcus, Clostridium histolyticum, Atopobium and Bacteroides populations were unmodified. Additionally, β-Lg:Gal glycoconjugate also significantly stimulated the growth of the Clostridium coccoides–Eubacterium rectal group. SC:Gal glycoconjugate gave rise to a significant increase in acetic acid concentration. From these results it could be inferred that the conjugation of proteins or peptides with non-prebiotic carbohydrates, such as Gal, might lead to new prebiotic products with different properties than the free carbohydrate or native protein.
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