Abstract
Summary Pistils were isolated from two pure lines of Petunia hybrida , the cyanidin type and 34dl0. The ovary wall was removed and the ovules were pollinated. By self- as well as by cross-pollination ca. 20–30 seeds per pistil were obtained. They showed 100% germinability and could be raised to flowering plants. In the case of cross-pollinations only hybrids were found. With these results the possibility of a haploid or diploid parthenogenesis is conclusively excluded. Partial removal of stigma and style does not impair test tube fertilization, but a complete amputation of the style has deleterious effects. Im competition experiments — pollen of different origin on stigma and ovules of the same pistil — the pollen put on the stigma had a much better chance for fertilization despite the longer way through the tissue of the style. The experimental data point out that test tube fertilization by sprenkling the pollen directly onto the ovules could be a way to overcome selfincompatibility in Petunia hybrida as well as in some other plants hitherto tested in this respect.
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