In vitro axillary shoot regeneration from cotyledonary node of Bishop’s weed (Trachyspermum ammi L.)

Asuman Koca,Muhammad Aasim

doi:10.30616/ajb.988363

Abstract

Bishop’s seed (Trachyspermum ammi L.) is a vital neutraceutial plant used as spice and also used as medicinal plant for curing different ailments and diseases of humans and animals. The study presents the in vitro regeneration of Bishop’s seed using cotyledonary node explant taken from in vitro germinated seeds. All mediums used in this study were comprised of MS (0.44%) medium having 3.0%sucrose and 0.65% agar with pH of 5.8. Expalnts were inoculated on MS medium enriched with 0.10-1.60 mg/L Thidiazuron (TDZ) alone or in combination with 0.10 mg/L IBA (Indole-3-butyric acid) for seven weeks under 16/8 light photoperiod provided with with cool-white fluorescent lamps. Thereafter, explants were sucultured on MS medium without any plant growth regulators (PGRs) for five weeks more under similar culture conditions. Results revealed 100% shoot regeneration frequency with 42.85-100.00%. The shoot count and shoot length ranged 3.43-19.40 and 1.04-2.74 cm respectively. The highest shoot count (19.40) and shoot length (2.74 cm) were observed on Ms medium enriched with 0.20 mg/L TDZ and 0.20 mg/L TDZ+0.10 mg/L IBA respectively. Relatively low rooting was recorded on IBA containing medium with low adaptation percentage of plantlets in pots containing peat moss.

Highlights

Bishop’s seed (Trachyspermum ammi L.) with common name of Ajowan, Ajwain, Carom or Ethopian cumin (Ashraf and Orooj, 2006) is one of the significant neutraceutial spice and medicinal shrub of Apiaceae or Umbelliferae family (Jeet et al, 2012)
This study presents the response of cotyledonary nodes explant cultured on mediums enriched with TDZ-Indole-3-butyric acid (IBA) for in vitro shoot proliferation of Bishop’s weed
The isolated explants were transferred immediately to MS medium enriched with variable concentrations (0.10, 0.20, 0.40, 0.80 and 1.60 mg/L) of Thidiazuron (TDZ) used alone or with combination of 0.10 mg/L of Indole-3-butyric acid (IBA)

Summary

Introduction

Bishop’s seed (Trachyspermum ammi L.) with common name of Ajowan, Ajwain, Carom or Ethopian cumin (Ashraf and Orooj, 2006) is one of the significant neutraceutial spice and medicinal shrub of Apiaceae or Umbelliferae family (Jeet et al, 2012). This study presents the response of cotyledonary nodes explant cultured on mediums enriched with TDZ-IBA for in vitro shoot proliferation of Bishop’s weed. Callus induction and shoot induction from cotyledonary node explant started simultaneously within two weeks of culture irrespective of PGRs type, concentration and combination.

Results

Conclusion