Abstract
To evaluate the effect of increasing storage time on the inhibitory effects of canine and feline plasma on matrix metalloproteinases (MMP) 2 and 9 in vitro. Matrix metalloproteinases 2 and 9 activity in the presence of canine plasma stored for 57, 155, 222, 316, 367, and 438days, and feline plasma stored for 17, 198, 565, and 954days was assayed using a commercially available colorimetric assay kit. For canine plasma, the MMP 2 activity for older samples was not significantly different than the 57-day sample (P=0.2025-0.9033). Two canine samples had significantly lower MMP 9 activity than the 57-day sample (367days: P=0.0099, 438days: P=0.0348, others P=0.0778-0.9928). For feline plasma, storage time did not significantly affect inhibition of MMP 2 and MMP 9 activity (ANOVA, P=0.2688 and P=0.2404, respectively). Increasing storage time does not significantly decrease the inhibiting activity of plasma on MMP 2 and 9 for up to 14months in dogs and 31months in cats.
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